Lesson 9 – Common Cell Culture Contaminants exam

1.Types of contamination that you cannot see with a standard inverted
microscope

A. bacteria

B. fungal

C. mycoplasma

D. None of the above

2.When looking for bacterial contamination, the best place to look is

A. in an area of the dish that is highly confluent

B. on the upper surface of the dish or flask since that is where the original contamination event occurred

C. between cells where there is a lighter back ground

D. over the whole dish at the lowest magnification possible

3.The atmosphere inside a biological safety cabinet is

A. full of CO2 gas

B. very clean but not absolutely sterile

C. absolutely sterile

D. lower in oxygen then the air outside

4.When working in the biological safety cabinet it is important to

A. make sure the cabinet is on and working correctly

B. Wipe the cabinet down with 70% alcohol

C. minimize quick movement

D. not introduce contamination

5.Of all the contaminations possible, probably the easiest to detect is

A. cross-contamination

B. yeast and fungi

C. mycoplasma

D. chemical

6.Mycelium is a form of

A. viral waste

B. Fungi

C. antibiotic

D. mycoplasm

7.In the event of a bacterial contamination the easiest thing to do is

A. discard your cells and thaw a frozen vial of fresh cells

B. keep culturing like nothing has happened

C. simply add antibiotics to kill the contaminant

D. wash the cells several times in D-PBS

8. Antibiotics should

A. always be used

B. Used only when necessary

C. never be used

D. only during a cross-contamination event

9. Following a bacterial contamination your media may turn yellow if there
is

A. L-Glutamine in the media

B. phenylalanine present

C. phenol red in the media

D. low CO2 in the media

10. LPS stands for

11. CPE stands for

12. stain is most commonly used for Mycoplasma
detection

13. are the commonly used antifungal
agents for cell culture use.

14. cell line caused wide spread cross contamination
throughout the world.

15. Serum is rich in nutrients and it is a primary cause for cell culture
contaminantion.

1. True

2. False

16. Researchers have to make an effort to increase the level of endotoxins
in cell cultures.

1. True

2. False

17. Mycoplasma is one of the most common contaminants, the detection
of which is very easy.

1. True

2. False

18. Mycoplasma is one of the most common contaminants, the detection
of which is very easy.

1. True

2. False

19. Mycoplasma is one of the most common contaminants, the detection
of which is very easy.

1. True

2. False

20. Aseptic control is able to totally eliminate biological contaminations in
cell culture.

1. True

2. False

21. What do you understand by cell culture contaminants? What are the
different types of cell culture contaminants?

22. What are the possible routes of contamination in cell culture?

23. What are the methods used for monitoring contamination?

24. How Mycoplasma contamination can be detection in cultured cell?

25. What are the general procedures for disposal of contaminated culture?

26. What are the causes of cell culture cross-contamination?

27. How you can control cell culture contamination in a cell culture
laboratory?