Lesson 8 – Differentiation of cultured cells exam

1. You are provided with an aliquot of embryonic stem cells derived from
goat. How would you proceed to drive the cells for osteogenic
differentiation and characterize them?

2. Paracrine effect refers to action of %BLANK% mediated by a
soluble factor %BLANK% the involvement of the systemic
vasculature

a) one cell on another adjacent cell, without

b) one cell on another adjacent cell, with

c) one cell on itself, without

d) one cell on itself, with

3. Morula is

a) unipotent

b) bipotent

c) pluripotent

d) totipotent

4. Which of the following statement is true except for transformed
cells?

a) low cell density favours differentiation

b) high cell density favours differentiation

c) high cell density favours proliferation

d) cell density does not influence differentiation or proliferation

5. Differentiation of a mammalian embryo that follows gastrulation is
regulated by

a) homotypic cell interaction

b) heterotypic cell interaction

c) paracrine factors

d) environmental factors

6. Differentiation of cells is a result of

a) activation of selected genes

b) inactivation of selected genes

c) both of the above

d) none of the above

7. Skin cells in culture can be induced to differentiate by

a) use of feeder layer

b) addition of EGF

c) air-liquid interphase

d) all of the above

8. Cells from one lineage acquiring the ability to differentiate into cells of
a different lineage is termed as

a) differentiation

b) dedifferentiation

c) transdifferentiation

d) undifferentiated

9. During liver regeneration, mature hepatocytes possess the following
differentiation properties:
i. can reenter the cell cycle and regenerate into hepatocytes
ii. liver cells have poor regenerative capacity
iii. satellite cells regenerate into hepatocytes

a) i is correct

b) ii is correct

c) both i and iii are correct

d) i, ii and iii are correct

10. expression is that phenotype expressed by a cell in
the absence of external regulation

11. A cell that can give rise to all known cell types or a whole organism is
said to be .

12. and cytokines regulate
erythropoiesis

13. is a lineage specific marker for epithelial cells

14. Adherent cells can be induced to grow as suspension cultures by
.

15. Schwann cells are characteristic of type of cells

16. refers to the loss of specific phenotypic properties of
cultured cells when compared to their in vivo counterparts from which
it was derived

17. Histotypic culture is a three dimensional culture that resembles the
in vivo tissue-like morphology

a) True

b) False

17. Stem cells in the basal layer of the epidermis are pluripotent

a) True

b) False

18. Irreversible differentiation in mature keratinocytes is marked by
cessation of DNA synthesis

a) True

b) False

19. Cellular environment is one of the factors that influence
differentiation

a) True

b) False

20. Cells upon transformation frequently exhibit aneuploidy

a) True

b) False

21. Selective overgrowth of undifferentiated cells of same lineage can
lead to dedifferentiation

a) True

b) False

22. Transglutaminase is a phenotypic marker for melanocyte

a) True

b) False

23. Vitamin A is a non physiological inducer of differentiation

a) True

b) False

24. Discuss on differentiation involving satellite cells

25. Lineage specific markers of differentiation

26. . Distinguish between transformation and transdifferentiation

27. Approaches for targeted differentiation of cultured cells

28. Non physiological inducers of differentitation

29. Types of differentiation in cultured animal cells and characterization

30. Regulation of differentiation in animal cells