Lesson 8 – Cryopreservation of Cells/Cell lines exam

1) The term ‘Cryopreservation’ was coined by

a) Montagazza

b) Allan Trounson

c) James Lovelock

d) Spallanzani

2) Cryopreservation of tissue is done at what temperature (ºC)?

a) 126

b) -80

c) -196

d) None of these

3) Which of the following is not used as a cryoprotective agent?

a) DMSO

b) Propylene glycol

c) Methanol

d) Polymers

4) What is the optimum temperature (ºC) for the revival of
cryopreserved cells?

a) 56

b) 72

c) 45

d) 37

5) What should be the required concentration range of a CPA?

a) 50-70%

b) 15-20%

c) 7.5-10%

d) None of these

6) Which CPA is preferred over glycerol for cryopreserving HL-60 cells?

a) 1, 2- propanediol

b) Hydroxyethyl starch

c) Sericin

d) Glycerol

7) Better post thaw recovery of suspension cells is achieved if the cells
were stored at -

a) Mid-log phase

b) Stationary phase

c) Lag phase

d) Log phase

8) Which freezing technique requires direct contact of straws with LN2

a) Controlled rate freezing

b) Rapid freezing

c) Vitrification

d) Slow freezing

9) What type of cryoprotectants (CPA) the DMSO is?

a) Cell membrane penetrating

b) Non-membrane penetrating

c) Both (a) and (b)

d) None of these

10) Which of the following is used in transporting the
cryopreserved cells or tissues?

a) LN2

b) Deep freezers

c) Ice packs

d) Solid CO2

11) ProFreeze is which kind of cell freezing media?

a) FBS containing

b) FBS-free

c) Excess FBS

d) None of these

12) What should be the material of the container where DMSO is
stored?

a) Silicon

b) Plastic

c) Polypropylene

d) None of these

13) Which of the following CPA is widely used in storage of animal
sperm?

a) DMSO

b) Polymers

c) Starch

d) Glycerol

14. observed that the maintenance of human spermatozoa
can be done at low temperatures

15. Protein can be used as a replacement of FBS in
cryoprotectant media

16. Concentration of DMSO used for animal cell preservation work is
%.

17. Examples of non-membrane penetrating cryoprotectants include

18. is the freezing process in which cell suspensions
are transformed directly from aqueous phase to glass state.

19. CPAs do not cause any damage to the cells/tissues

1. True

2. False

20. Glycerol is less toxic than any other cryoprotective agents.

1. True

2. False

21. The cryopreservation medium can be kept at 4°C for upto many
months.

1. True

2. False

22. There is no harm to the viability of the cells undergoing repeated
freeze-thaw.

1. True

2. False

23. The main aim of cryopreservation is to bring the cells at zero
metabolism and non-dividing state at low temperature in the
presence of CPA

1. True

2. False

24. Explain in brief about programmable cell freezer

25. What are the basic steps in the cryopreservation of mammalian
cells?

26. Explain about freezing media and its types

27. What are cryoprotectants?

28. Write in brief the advantages and disadvantages of cryopreservation

29. Write about the steps involved in cryopreservation of cell line.

30. Explain about the different freezing processes used nowadays for
cryopreservation.