Lesson 7 – Estimation of cell concentration – Indirect methods exam

Welcome to your Lesson 7 - Estimation of cell concentration – Indirect methods exam

1. The total number of viable cells in a culture of vero after
trypsinization was estimated to be 2.3 x 106
cells/ml by trypan blue
exclusion assay. The culture requires to be seeded onto 6-well plate
@ 1ml/well and accordingly it was diluted 1:23 in culture medium
and then seeded onto the multi well plates. Calculate the final
seeding cell density per well?
2. The total number of viable cells in a culture is found to be 7.5 x
106
cells/ml. The cells are to be cultured on to a 24-well plate that
requires 1.5 x 105
cells/ml. How much should the cell suspension be
diluted so that 500 μl can be added to each of the wells?
3. Which of the following assay is not useful to determine protein
content?
4. Which of the following is / are metabolic assay(s) for quantifying cell
viability based on mitochondrial enzyme activity?
5. Propidium iodide is a membrane %BLANK% fluorescent dye
and is used to stain %BLANK% cells
6. Cellular dehydrogenases reduce tetrazolium salts to formazan, the
change in colour reaction being %BLANK% in viable cells
7. Viable cells fluoresce %BLANK% when fluorescent
diacetate is excited with blue light
8.Necrotic cell death can be assessed by %BLANK% markers
i. LDH
ii. IL-1β
iii. JC-10
9. Indirect method of cell count through quantification of ATP in cultured
cells can be achieved by
10. Colony forming assay is an indicator of %BLANK% count of
cultured cells
11. Bradford assay is used to determine the content of
the cultured cells and the absorption maximum is at nm wavelength
12.In Trypan blue dye exclusion method of staining, %BLANK% cells
take up the blue colour of the stain
13. Viable cells reduce the terazolium salts by the action of
enzyme to form (name of the
product)
14. Hoechst emits %BLANK% colour fluorescence upon staining
15. The rate of DNA synthesis is measured by estimating the tritiated
thymidine detected by %BLANK% .
16. In colony forming assay, each represents
that grew from a sample population
17. In Bradford assay, when protein in the cell sample binds to
Coomassie dye the change of colour is from red to blue
18. MTT is a derivative of tetrazolium iodide
19. Indirect methods of determination of cell concentration are more
accurate than direct methods
20. Hoechst dyes are membrane impermeable dyes used for nuclei
staining
21. As per Beer-Lamber’s law, an inverse relationship exists between
absorbance and concentration of the solution
22. Neutral red is used as a vital stain for staining lysosomes of cultured
cells
23. The rate of DNA synthesis can be determined by measuring the
incorporation of 3H-leucine in cultured cells
24. The incubation temperature at which cells are handled and reacted
with enzyme is an important criteria while determining LDH leakage
25. Dye exclusion method of testing viability of cultured animal cells
26. Estimation of cell concentration using DNA content of cultured cells
27. What is MTT assay? Briefly narrate the improved versions (substrates)
of MTT assay and their advantages over MTT
28. LDH leakage and its significance in cultured cells
29. Fluorescent based detection systems for determining viability of
cultured cells
30. Colony forming assays for viability assessment
31. Indirect methods available for determination of cell count of cultured
animal cells, their significance and constraints
32.List out the various techniques adopted for assessing the viability of
cultured cells. Describe in detail any one technique that is followed in your
laboratory.