Lesson 5 – Serum free media exam – Brihha Assessment Platform

1. Disadvantage of serum free media is

a. It is highly specific to one cell type

b. Require addition of different substances according to cell type

c. Both of the above

d. None of the above.

2. Cell culture medium without serum has the
following advantages expect

a. There is no batch variation of the serum

b. Variety of cells can be cultured in serum free medium

c. Ultra high purity of reagents are required

d. Less protein interference in bioassay

3. Serum free media has the following advantages:

a. It is highly specific to one cell type, so additional and different selective media will have to be used for each cell type.

b. Reliable serum free media readily available commercially.

c. Ultra high purity of reagents, water and critical control ofpH and temperature are required

d. all of the above

4. Examples for reductants used in serum free media

a. Tween 80

b. Mercaptoethanol

c. Pluronic F-68

d. carboxy methyl cellulose

5. Harmone necessary for MDCK cells proliferation

a. Hydrocartisone.

b. Insulin.

c. Growth harmone.

d. Triiodothyronine

6. Cells, which will grow, survive, or maintain function only when attached
to a surface such as glass or plastic are .

7. is an important component of serum free media that promote
attachment of cell to glass substrate

8. is used for adhesion of epethelial cells.

9. enhaces plating efficiency in different types of cells

10. begun the total omission of serum and protein in cell culture
media

11. Knockout Serum Replacement (KSR), is a formulation of various
active ingredients of serum.

1. True

2. False

12. Cells grown in vivo are always regulated by somatomedin and
hormones

1. True

2. False

13. High pH causes dissociation of Fe3+ from ferro-transferrin; the
apotrasferrin so formed is returned back to outside cell

1. True

2. False

14. Clonal growth of a strain of Chinese hamster cells requires
supplementation of spermidine, spermine and putrescine

1. True

2. False

15. Cell growth is often faster with higher saturation density with serum
free media

1. True

2. False

16. Write the composition of commonly used commercial serum free
media?

17. List the advantages of serum free media?

18. . Explain the constituents of a serum free media?

19. Explain the brief historical events that led to development of serum free
media?