Lesson 5 – Secondary cell culture exam – Brihha Assessment Platform

1. In secondary cell culture:

(a) Cell is cultured directly from intact animals

(b) Cell is cultured from primary cell culture

(c) Both of the above.

(d) None of the above.

2. Finite cell lines exhibit the property of

(a) contact inhibition

(b) density limitation

(c) anchorage dependence.

(d) All of the above

3.Continuous cell lines show the property of

(a) aneupliody or heteroploidy

(b) absence of contact inhibition

(c) Both of the above.

(d) None of the above

4.When the cells in a primary culture stop growing a new culture may
be established by inoculating some of the cells into fresh medium. This
is called

(a) Subculturing

(b) Passaging

(c) Both of the above

(d) None of the above

5.Finite cell lines have cell generations of

(a) 20-80 population doublings

(b) 50-100population doublings

(c) 20-50population doublings

(d) 200-300population doublings

6.Following are the properties of primary culture

(a)finite life span

(b)difficulty in maintainance.

(c)close resemblence to parental tissue

(d)all of the above.

7. The basal media formulations enriched with nutrients and animalderived factors is

(a)basal media

(b)serum free media

(c)reduced serum media

(d)all of the above

8.Prolonged life span of transformed cells is due to

(a)Periodic subculturing

(b)Transformation

(c)Genetic alteration

(d)all of the above

9. When a primary culture is sub-cultured, it is known as

10. A monolayer of cells in which all cells are in contact with other cells all
around their periphery, and no available substrate is left uncovered is called

11. The enzyme will break both the bonds holding the cells
together and those involved in attachment to the surface

12. The transfer of cells from one culture vessel to another, involves the sub
division of a proliferating cell population, enabling the propagation of a cell
line or cell strain is

13. A propagated culture after the first subculture is

14. On the basis of the life span of culture, the cell lines are categorized into
two types and

15. are specifically designed for suspension cell culture known
for superior gas exchange and permit higher volumes of cells to be cultured.

16. media requires supplementation of serum.

17. In secondary cell culture cell is cultured directly from intact animals.

1. True

2. False

18. A secondary culture is established after the first passage of the primary
culture.

1. Ture

2. False

19. Cell lines transformed under laboratory conditions or in vitro culture
conditions give rise to continuous cell lines.

1. True

2. False

20. Growth medium used to propagate a particular cell line; usually a basal
medium with additives such as serum or growth factors.

1. True

2. False

21. Serum will break both the bonds holding the cells together and those
involved in attachment to the surface.

1. True

2. False

22. Subculturing of suspension cells is more complicated than adherent cells

1. True

2. False

23. Cloning efficiency of finite cells is higher than continuous cells

1. True

2. False

24. Serum-free media (SFM) circumvents using animal sera by replacing the
serum

1. True

2. False

25. WHAT IS SECONDARY CELL CULTURE?

26. EXPLAIN THE PROCESS OF SUBCULTURE?

27. .DEFINE MEDIA AND TYPES OF MEDIA COMMONLY USED?

28. .DEFINE CELL LINE?

29. WHAT IS THE IMPORTANCE OF SERUM IN CELL CULTURE?

30. Differentiate between finite and continuous cell line?

31. Differentiate between primary and secondary cell culture?