Lesson 4 – Morphology of cultured cells exam

1. Prepare an e-(colour) atlas on morphology of cultured animal cells with
pictures of various primary cultures and cell lines

2. The type of cultured cells with spindle shaped appearance are

a) epithelial

b) fibroblast

c) lymphoblast-like

d) adipose

3 Cell line of rat liver origin is

a) BRL

b) L6

c) MDCK

d) BHK

4. Regarding neuronal cells, which of the following is incorrect?

a) Type I cells do not have axons

b) Type II cells have long axons

c) Type I cells have long axons

d) Type II cells have short axons

5. Fibroblast overgrowth means that fibroblasts that exhibit relatively
%BLANK% population %BLANK% doubling times the other
cells in a primary culture after a few generations .

a) long, outgrow

b) long, are outgrown by

c) short, are outgrown by

d) short, outgrow

6. Which of the following is not a cytopathic effect in cultured cells?

a) vacuolation

b) syncytial formation

c) formation of inclusion body

d) mitotic spindle formation

7. Morphometry is a term used to describe

a) the shape of the cell

b) the size of the cell

c) the motility of the cell

d) the healthiness of the cell

8. COS-1is a %BLANK% cell line of %BLANK% type
derived from pig kidney

a) continuous, fibroblast

b) finite, epitheloid

c) continuous, epitheloid

d) finite, fibroblast

9. Morphology of the cells in culture is influenced by
i. cell density
ii. alterations in the substratum
iii. addition of supplements to the medium

a) i is correct

b) ii is correct

c) both i and ii are correct

d) i, ii and iii are correct

10. The formation of at confluence is characteristic
of adherent cells

11. type of cells (morphology) exhibit cobble stone
appearance

12. Hanging drop technique of cell culture is adopted for
culture

13. In suspension cultures, high cell density result in of cells.

14. The CHO cell line is type of morphology derived from
the of Chinese hamster

15. Dendrites are characteristic of type of cells

16. Morphology of live cells under culture can be best viewed using
microscope.

17. HeLa is the first human derived cancerous cell line

a) True

b) False

18. Observation of morphology of cultured cells is best done
immediately after trypsinization

a) True

b) False

19. Liver cells require special culture systems to regain their polarity and
express their phenotypic characteristics

a) True

b) False

20.Morphology of the blastomeres is one of the criteria for determining
the quality of embryos

a) True

b) False

21. Cells upon transformation frequently become anchorage dependant

a) True

b) False

22. MDCK is an epithelial type cell line derived from liver of dog origin

a) True

b) False

23. Nuclear to cytoplasmic ratio is high in stem cells

a) True

b) False

24. Illustrate with an example how morphology of cultured cells gets altered
due to variations in cell density

25. Illustrate with an example how morphology of cultured cells gets altered
due to variations in cell density

26. Cytopathic changes in cultured cells

27. Significance of monitoring morphology of cultured cells

28. Cover slip cultures

29. Different cell types based on morphology of cultured cells with
illustrations

30. Factors affecting the morphology of cultured cells