Lesson 4 – Importance of serum in cell culture media exam

1. Addition of serum in media has following advantages, except:

(a) May protect against proteolytic action of trypsin

(b) May contain inadequate level of cell specific growth factor

(c) Acts as carrier of many water soluble nutrients

(d) Cell is protected in agitated cell culture

2. Addition of serum in media is advantageous, because:

(a) It provides peptides and other growth regulating hormone

(b) It is used to increase cell mass

(c) Provides essential low molecules of nutrients

(d) All of the above

3. The disadvantage of serum in culture medium are

(a) Batch to batch variation

(b) Contamination

(c) Increases the viscosity of the medium

(d) both a and b

4. Growth factor that regulates differentiation and act as
inductive signals in embryonic development.

(a) TGF

(b) IGF

(c) FGF

(d) PDGF

5. Heat inactivation of is done at following temperatue

(a) 52°C – 10 minutes

(b) 55°C -30 minutes

(c) 56°C- 30 minutes

(d) 56°C - 20minutes

6. The serum used in culture medium is generally obtained
from

7. Newborn calf serum is processed from animals that are under of age.

8. Example for growth Factors present in Serum

9. Fetal bovine serum contains low levels of which have a cell
growth-inhibitory activity

10. 10% (v/v) foetal calf serum contains approximately
fibronectin/ml

11. Fibroblast growth factor is a major growth factor present in serum

1. True

2. False

12. Albumin is a major protein present in serum

1. True

2. False

13. Sera should be stored at temperatures above −20°C

1. True

2. False

14. Albumin is involved in transport of iron

1. True

2. False

15. Serum anti-protease activity arrests proteolysis after trypsinisation

1. True

2. False

16. Write the importance of using serum in cell culture media ?

17. List the disadvantages of using serum?

18. Describe the components of serum in detail