Lesson 4 – Antibody of Immunoglobulin exam

1. B cells that produce and release large amounts of antibody are called

a) Memory cells

b) Basophils

c) Killer cells

d) Plasma cells

2. Paratope is an

a) Antibody binding site on variable regions

b) Antibody binding site on heavy regions

c) Antigen binding site on variable regions

d) Antigen binding site on heavy regions

3. The specificity of an antibody is due to.

a) Variable portion of the heavy and light chain

b) Heavy chains

c) Fc portion of the molecule

d) Its valence

4. All of the following are true about antibodies, EXCEPT which one?

a) They occur on the surface of B-lymphocyte

b) They predominate the primary immune response to antigen

c) They are glycoproteins

d) They are molecule with a single, defined amino acid sequence

5. The major immunoglobulin family to which a particular immunoglobulin
belongs can be determined by sequential analysis of the 110 amino acids
beginning from the

a) Amino terminus of the heavy chain

b) Carboxy terminus of the heavy chain

c) Amino terminus of the light chain

d) Carboxy terminus of the light chain

6. All of the following are true EXCEPT

a) An antigenic determinant is a paratope

b) The class of an immunoglobulin is determined by its heavy chain

c) An epitope is a small portion of a macromolecule

d) An IgG antibody is bivalent

7. Which of the element must be found in antibodies

a) Sulphur

b) Phosphous

c) Sodium

d) Copper

8. Antigen-binding domain contributes to which of the following
characteristics of antibodies

a) Superiority only

b) Selectivity only

c) Selectivity and Specificity

d) Superiority and Specificity

9. Class-specific antigenic determinants (epitopes) of immunoglobulins
are associated with

.

a) L chains.

b) J chains.

c) Disulfide bonds

d) Variable regions.

10. The idiotype of an antibody molecule is determined by the amino acid
sequence of the

a) Constant region of the L chain.

b) Variable region of the L chain.

c) Constant region of the H chain.

D) Variable regions of the H and L chains.

11. A polyclonal antiserum raised against pooled human IgA will react with

.

a) Human IgM.

b) Light chains.

c) Human IgG

d) J chain

12. The ability to make antibody with the same antigen specificity but
different Fc regions

A) Causes allelic exclusion of Ig molecules.

B) Does not occur against bacterial antigens.

C) Improves the antigen binding specificity of an Ig molecule.

d) Increases the effector functions of Ig molecules

13. Allergy symptoms are produced when antigen binds to IgE on FcR on

A. Cells.

B. Macrophages

C. Mast cells

D. Neutrophils

14. Papain digestion of an IgG preparation of antibody specific for the
antigen hen egg albumin (HEA) will

a) Lose its antigen specificity.

b) Precipitate with HEA.

c) Lose all interchain disulfide bonds.

d) Produce two Fab molecules and one Fc fragment

15. The first immunoglobulin synthesized by the foetus is

a) IgA.

b) IgE.

c) IgG.

d) None; the fetus does not synthesize immunoglobulins.

16. The primary and secondary antibody responses differ in

a) The predominant isotype generated

b) The number of lymphocytes responding to antigen

c) The speed at which antibodies appear in the serum

d) All of the above

17. Antibodies in adult animal are formed by

a) Mast cells

b) Macrophages

c) NK cells

d) Plasma cell

18. J chain is present in

a) IgM

b) IgY

c) IgE

d) IgG

19. Clonal selection theory was proposed by

a) Jerne

b) Alexander

c) Ehrlich

d) Burnet

20. Which of the following statements concerning papain cleavage of IgG
is incorrect?

a) Fc fragment contains part of the heavy chain

b) Fab fragment contains both heavy-chain and light-chain determinants

c) Fc fragment interacts specifically with certain lymphoid cell receptors

d) Fab fragment is divalent

21. The antigen-binding sites of an antibody molecule are located in the

a) C region of light chains

b) V region of light chains

c) C region of heavy chains

d) regions of both light and heavy chains

22. The class-specific antigenic determinants of immunoglobulins are
associated with the

a) Light chain

b) Heavy chain

c) J chain

d) Secretory component

23. The greater resistance of secretory IgA to proteolytic enzymes is
assumed to be a consequence of the

a) Binding of the secretory component

b) Predominantly dimeric nature of secretory IgA

c) Antiprotease activity of the heavy chain

d) Presence of the J chain

24. Idiotypes are

a) Located in the C region of the light chain

b) Located in the C region of the heavy chain

c) Located in and around the antigen-binding region of an antibody molecule

d) None of these.

25. The fusion of what cells leads to hybridoma cell line that secrets
monoclonal antibodies?

a) Plasma cells and myeloma cells

b) Monocytes and lymphocytes

c) Lymphocytes and lymphoma cells

d) Plasma cells and activated T cells

26. An antibody Fab contains

a) Complementarity determining regions

b) H and L chain variable regions

c) One antigen binding region

d) All of the above

27. The regions of the antibody molecule which contribute most to the
affinity of the antibody for antigen are the

a) CDR

b) Fab regions

c) Fc regions

d) Framework region

28. Antibody Fc fragments contain
a) Antigen-binding sites

c) Complement-binding sites

b) CDR

c) Complement-binding sites

d) Framework residues

29. The immunoglobulin isotype is determined by the

a) Antigen specificity.

b) H chain constant region.

c) L chain variable region.

d) Number of antigen-binding sites.

30. Which statement about antigen epitopes is FALSE?

a) An epitope may be shared by two different antigens.

b) A protein molecule usually contains multiple epitopes.

c) B cells bind only processed antigen epitopes.

d) Epitopes may be linear form by protein folding in primary amino acid sequence.

31. An example of an antigen epitope from an infectious organism would
be

a) A bacterial endotoxin (LPS) molecule.

b) A fungal cell wall protein.

c) A peptide on the surface of a virus capsid protein.

d) A whole virus.

32. Antibody affinity for antigen depends on

a) Antibody isotype

b) Complementary shape each antibody V region for its antigen epitope

c) Number of Fab regions in each antibody molecule

d) Whether the antibody is in the serum or on the cell surface

33. Avidity

a) Is a pathogenic agent, causing a very serious disease

b) Occurs when the ratio of antibody to antigen is optimal

c) Refers to the strength of interactions between a multivalent antibody and a multivalent antigen

d) Results in a loss of antibody reactivity

34. Allotypic determinants are

a) Constant region distinguishs each Ig class and subclass within a species.

b) Expressed only from the paternal chromosome.

c) Generated by the conformation of antigen-specific VH and VL sequences.

d)Amino acid differences due to different alleles for the same H or L chain locus.

35. Plasma cells produces .

36. Papain breaks immunoglobulin molecule into and .

37. The four chains of immunoglobulin are held together by a combination. Non-
of and bonds.

38. The variable region domains namely and are involved in
binding of antigens.

39. The defines how a specific antibody will contribute to an
immune response. Constant region or Fc region

40. The is a flexible amino acid stretch in the central part of the
heavy chains of the IgG and IgA immunoglobulin classes.

41. The hypervariable regions on the variable chain of antibody is called as
.

42. Hinge region is rich in and amino acids.

43. Immunoglobulin possesses and types of light chains.

44.Antibodies are reacting with epitopes found on a wide variety of
unrelated molecules is called as .

45.The is the tail region of an antibody that interacts with cell surface receptors called Fc receptors.

46.The basic structural unit of an antibody molecule consists of
polypeptide chains.

47. Any immunoglobulin, both light and heavy chains have a variable
sequence at their ; but a constant sequence at their .

48. Immunoglobulin domain

49. Constant region

50. Variable region

51. Hinge region

52. Disulfide bond

53. FV fragments

54. Superantigens (SAgs)

55. Paratope

56. Idiotype

57. Isotype

58. Hypervariable (HVR) or Complementarity Determining Regions (CDR)

59. Framework regions

60. Draw a labeled diagram of IgA molecule and mention its characters and
functions.

61. What do you mean by diversity of antibody? Briefly explain mechanism
of it.

62. Write a brief essay on the 'primary structure of immunoglobulins'

63. Antibodies are Y-shaped molecules formed by four protein chains which have similar length.

A) True

B) False

64. Epitopes are the specific sites on the antibodies which can bind to antigen molecules.

A) True

B) False

65. The hinge region of the antibody is a short stretch of amino acids on the heavy chain located between the chain’s CH1 and CH2 regions.

A) True

B) False

66. Every immunoglobulin molecule has at least two antigen binding sites, which are identical to one another.

A) True

B) False

67. Heavy chain constant domains including CH1 are responsible for the special biological properties of immunoglobulins.

A) True

B) False

68. The hinge region of an antibody molecule improves the efficiency of antigen binding and cross-linking.

A) True

B) False

69. The basic structural unit of antibody molecule consist of two identical light chains and two identical heavy (H) chains. A) True B) False

A) True

B) False

70. Either lambda or kappa chains are present in all immunoglobulins.

A) True

B) False

71. The two heavy chains in the immunoglobulin molecule are held together by disulphide bond.

A) True

B) False

72. Immunoglobulins are polysaccharides.

A) True

B) False

73. In cattle maternal antibodies are transferred to progeny via colostrums.

A) True

B) False

74. The variable region of immunoglobulin is involved in antigen binding.

A) True

B) False

75. Complement binding occurs in the Fc portion of immunoglobulins.

A) True

B) False

76. Antibodies are protein molecules produced by plasma cells.

A) True

B) False

77. Immunoglobulins constitute 20-25% of the total serum proteins.

A) True

B) False

78. Hot spots are present in variable region.

A) True

B) False

79. The variable region of immunoglobulin molecules are involved in binding with antigens.

A) True

B) False

80. Incomplete antibodies are known as blocking antibodies.

A) True

B) False

81. Maternal antibodies give protection for more than one year.

A) True

B) False

82. Immunoglobulins are glycoproteins

A) True

B) False

83. Antigenic determinant site is present on the surface of antibody.

A) True

B) False

84. The antibodies rose against the ?-? T cell receptor are called clonotypic antibodies.

A) True

B) False

85. Prepare a suitable model for different domains of immunoglobulin
with their function.