Lesson 3 – Growth supplements exam – Brihha Assessment Platform

1. Number of essential amino acids that should be supplemented in cell
culture

a. 6

b.13

c. 8

d. 10

2. Requirement of glutamine compared to other amino acids is

a. 20-40 fold greater

b. 5-10 fold greater

c. 3-40 fold greater

d. 2-3 fold greater

3. Growth factor involved in carbohydrate metabolism

a. PDGF

b. FGF

c. EGF

d. IGF

4. The hormonal supplement involved in improving the cloning
efficiency of the glial cells and fibroblasts.

a. Hydrocortisone

b. Thyroxin

c. Growth hormone

d. Insulin

5. The growth factor with multifunctional mitogenic activity is

a. IGF

b. FGF

c. EGF

d. PDGF

6. Commonly used growth supplement is

7. Growth factor rich in serum

8. Most critical essential amino acid in cell culture is

9. Example for haemopoietic growth factor

10. Horse serum has lower concentration of which form
polyamine

11. Fibroblast growth factor (FGF) is involved in proliferation of
fibroblast cell, differentiation and senescence in vitro

1. True

2. False

12. IGF is not a good mitogen for chicken embryo fibroblast

1. True

2. False

13. Survival of low-density cultures could be improved by growing the
cells in the presence of feeder layers

1. True

2. False

14. BactoPeptone and tryptose are examples of animo acid hydrolysates

1. True

2. False

15. NGF does not promotes survival and nerve process
outgrowth of s neurons

1. True

2. False

16. Describe the importance of serum as a growth supplement ?

17. Explain the principle of feeder layers and conditioned media?

18. Describe about different types of growth supplements and their
importance in cell culture ?