Lesson 3 – Development of Cell Lines: (C) Development of Stable Cell Lines for Large Scale Production of Recombinant Proteins and other Biopharmaceutical Products exam

Welcome to your Lesson 3 - Development of Cell Lines: (C) Development of Stable Cell Lines for Large Scale Production of Recombinant Proteins and other Biopharmaceutical Products exam

1.How adherent cell culture can be adapted to suspension cultures?
Explain with examples

2. What types of expression vector systems are currently available
commercially for large scale production of biopharmaceutical
products?

3. Transient gene expression remains for:

a) Longer time

b) Short time

c) Life time

d) Few months

4. Which cell line is commonly used as stable cell line for large scale
production of biomolecules:

a) HeLa

b) HCT

c) CHO

d) MDCK

5. Which antibiotic can be used for selection of the mammalian
cellsfollowing transfection with a mammalian expression vector:

a) G418

b) Zeocin

c) Hygromycin

d) All of the above.

6. Most popular chemical transfection method for stable cell line
development is:

a) DEAE dextran based

b) Cationic lipid based

c) CaCl2 based

d) none of the above

7. Kill curve prepared for selection drug determines:

a) Maximum amount of drug to kill cells

b) Minimum amount of drug to kill cells

c) Both

d) None of the above

8. Stable cell lines allows expression of recombinant protein for:

a) for prolonged period

b) Short term

c) for limited passages

d) None of the above

10.In rDNA technology Selection antibiotic is used to select:

a) Recombinant cells

b) Non-recombinant cells

c) to kill the recombinant cells

d) none

11. Recombinant protein production involves the use of

a) Vector

b) Gene of interest

c) Expression host

d) All of above

12. The process of insertion of recombinant DNA into animal cell is
called as:

a) Transduction

b) Transfection

c) Conjugation

d) Transformation

13.Which of the following viral vectors is unable to integrate into
host genome

a)Retroviral vector

b) Adenoviral vector

c) Lentivirus vector

d) Adeno associated viral vector

14. Recombinant DNA is allowed to multiply and express the transgene
in a .

15. Plasmid vector copy numbers within a competent host cells depends
on the .

16. is an example of selection antibiotics which can act
against insect, mammalian cells, bacteria and yeast cells.

17. Integration of recombinant DNA into the host genome is a must for
development of a cell line.

18. In a DHFR deficient CHO cell line, selection pressure is created by
using the drug

19. A complex human gene can be efficiently expressed in E. coli with
post translation modifications

True

False

20. Electroporation is only suitable for stable transfection.

True

False

21. All expression vectors can be used for development of stable cell
lines.

True

False

22. Lentiviruses are a subclass of retroviruses that can integrate into the
genome of non-dividing cells.

True

False

23. Constitutive protein production for prolong periods needs stable
integration of gene of interest into the genome of the host cell.

24. Write a short note on the selection of recombinants with an example.

25. Describe the properties of expression host in recombinant DNA
technology.

26. What is Gene optimization and how it is important for recombinant
protein expression?

27. What is single cell cloning? Write in brief.

28. What is Viral promoter?

28. Write a short note on the selection of recombinants with an example.

29. Describe the properties of expression host in recombinant DNA
technology.

30. What is Gene optimization and how it is important for recombinant
protein expression?

31. What is single cell cloning? Write in brief

32. What is Viral promoter?