Lesson 3 – Basic requirements of a cell culture laboratory exam

1. Cryopreservation of cell culture is done at:

(a) -180°C in liquid nitrogen

(b) -120°C in deep freeze

(c) + 4°C in an ordinary refrigerator

(d) None of the above.

2. Scaling of cell culture is done to:

(a) Enhance the quantity of cell culture

(b) Enhance the quality of cell culture

(c) Both of the above

(d) None of the above.

3. Scaling up of suspension culture is done by :

(a) Airlift method

(b) By stirring method

(c) Both of the above

(d) None of the above.

4.Scaling up of anchorage dependent culture is done by:

(a) Roller tube method

(b) Microcarrier system

(c) Hollow fiber system

(d) All of the above

5. In microcarrier system cells:

(a) Grows on monolayer on the surface of small spheres

(b) Grows as monolayer on the surface of Roux flask

(c) Grows as monolayer in the glass culture tube

(d) All of the above

6. An example of a microbe that is typically worked with at a BSL-2
laboratory is

(a) Staphylococcus aureus.

(b) non-pathogenic strain of E. coli

(c) Mycobacterium tuberculosis

(d) none of the above

7. Laboratory practices in BSL-4 includes

(a) Change clothing before entering.

(b) Shower upon exiting.

(c) Decontaminate all materials before exiting.

(d) All of the above

8. IBSC

(a) International Bio-Safety Committee

(b) Institutional Bio-Safety Committee

(c) Indian Bio-Safety Committee

(d) All of the above

9. The percentage of CO₂ and temperature of incubator used for cell culture
is

10. microscope is used for microscopic examination of cells in cell
culture.

11. Chemical sterilization done by

12. instrument used to check the quality of triple distilled water

13. Preservation of seed and using stocks of cell line by

14. An example of a microbe that is typically worked with at a BSL-1 is

15. A microbe that is typically worked with in a BSL-3 laboratory is

16. The microbes in a lab are dangerous and exotic, posing a high risk
of aerosol-transmitted infections.

17. Class II vertical laminar air flow cabinet and this type of cabinet is used
widely in cell culture laboratories.

1.True

2.False

18. Other filters of a smaller pore size are also available to allow the removal
of mycoplasma 0.45micrometer.

1. True

2. False

19. Low temperature (< 80°C) freezer storing unstable reagents
;freezingcryovials in insulated container.

1. True

2. False

20. The advantage of microcarrier system is high yield of cells from smaller
culture volume.

1. True

2. False

21. Gamma-irradiation of 1.5 Mrad (C0 60 source) are employed for
plasticware sterilization

1. Ture

2. False

22. High risk biological agents is seen in BSL-4

1. True

2. False

23. Microbes worked with in a BSL-4 laboratory includes Ebola

1. True

2. False

24. In a BSL-1 lab, the microbes present high levels of potential hazard to
laboratorians and the environment

1. True

2 False

25. Explain the different biosafety level cabinets

26. What are the different disposable plasticware used in a cell culture
laboratory?

27. Explain the importance of roller apparatus.

28. What are cell scrapers?

29. What is the importance of water purification in a cell culture lab?

30. Visit the cell culture laboratory of your institution and draw the layout
of it

31. Categorize the most common equipment used in a cell culture lab