Lesson 2 – Cell cycle and Control of Proliferation of cultured animal cells exam

1. Prepare a slide show detailing the double thymidine blockade used for
synchronization of cultured cells followed by cell cycle analysis

2. Which of the following cells do not lack the ability to divide?

a) skin cells

b) nerve cells

c) muscle cells

d) red blood cells

3. Cells that have stopped dividing and are arrested in a state preceding
that of DNA synthesis, are said to be in the %BLANK%

a) S phase

b) G1 phase

c) G0 phase

d) G2 phase

4. For adherent cells, adhesion provides a signal for cells to

a) grow and divide

b) transduce differentiation signals

c) enhance survival

d) all of the above

5. A mutation in the gene for a cyclin protein might result in which of
the following?

a) a cell with additional genetic material than normal

b) cancer

c) a cell with less genetic material than normal

d) any of the above

6. What is a primary function of tumor suppressor genes?

a) stop all cells from dividing

b) stop certain cells from dividing

c) help oncogenes produce oncoproteins

d) allow the cell to skip certain phases of the cell cycle

7. During mitosis, the cell becomes %BLANK% to external stimuli

a) highly responsive

b) less responsive

c) unresponsive

d) none of the above

8. Apoptosis is characterized by

a) DNA fragmentation

b) blebbing

c) formation of apoptotic bodies

d) all of the above

9. High cell density %BLANK% proliferation of cells

a) promotes

b) inhibits

c) does not influence

d) none of the above

10. Proliferation of fibroblasts is aided by %BLANK%
i. FGF
ii. EGF

a) i is correct

b) ii is correct

c) both i and ii are correct

d) neither i nor ii is correct

11. DNA replication can be monitored by incorporation of
.

12. DNA replication occurs during of the cell cycle.

13. The entry of a cell into M phase is initiated by .

14. Cyclin binding leads to a change in the of kinases
to initiate its activity

15. Cellular proliferation is maximum in the phase of the
growth curve

16. Colcemid is used as a chemical blockade for arresting at
phase of the cell cycle

17. The is regarded as guardian of the genome and is
involved in cell arrest

18. Cessation of cellular proliferation is termed as %BLANK% .

Cytostasis

19. Liver cells do not usually divide but can be induced to divide.

a) True

b) False

20. Rb is a tumor suppressor gene and not an oncogene

a) True

b) False

21. Mitosis is the process of segregation of DNA molecules into two cells

a) True

b) False

22. Telomeres get shortened progressively in actively dividing cells

a) True

b) False

23. G1 phase is characterized by preparation for DNA synthesis

a) True

b) False

24. Erythropoietin is essential for proliferation of epithelial cells.

a) True

b) False

25. Primary cultures do not exhibit density limitation of cell proliferation

a) True

b) False

26. Aminopterin is an example of S-phase blocking agent

a) True

b) False

27. Significance of Go / G1 phase of cell cycle.

28. Difference between proliferation and transformation

29. Inducers of proliferation of cultured cells

30. Chemiluminescent detection of rate of DNA synthesis as a measure of cell proliferation

31. Flow cytometry for determination of the phase of cell cycle

32. Phases of cell cycle and appropriate synchronization protocols

33.Cell proliferation assays