Lesson 1 – Introduction and importance of cell culture exam

1. The two major branches of medical research of cell culture
importance

a) Biopharmaceuticals and vaccine production.

b) production of antiviral vaccines and the understanding of neoplasia

c) model system and cancer studies.

d) drug screening and vaccine production

2. The maintenance of whole organs or fragments of tissue is termed as

a) Histotypic celture

b) Cell culture

c) organ culture

d) None of the above

3. Applications of Cell Culture Studies in Pharmaceutical Technology
includes

a) drug screening

b) cancer studies

c) toxicity studies

d) all of the above

4. Advantages of cell culture

a) Control of physicochemical environment

b) Cell cultures reduces the use of animals for various experiment

C) Cultured cells can be stored in liquid nitrogen for several years

d) All of the above

5. Limitations of the cell culture

a) high capital investment

b) need necessary expertise and technicalities

c) heterogeneity of continuous cell lines

d) all of the above

6. A monolayer of cells in which all cells are in contact with other cells all
around their periphery and no available substrate is left uncovered

a) confluency

b) subculture

c) primary culture

d) none of the above

7. For a transgenic system to work, the virus to be detected must be

a) able to adhere to the cell wall

b) prime its replication cycle without reaching the finishing point

c) replication cycle must beadequate to activate the gene through the promoter

d) all of the above.

8. Biologicals produced by recombinant DNA (rDNA) technology in
animal cell cultures

a) anticancer agents, enzymes,

b) immunobiologicals

c) hormones.

d) All of the above.

9. refers to the isolation of cells from an animal or plant
and their subsequent growth in a favorable artificial environment.

10. A subpopulation of a cell line is positively selected from the culture
by cloning or some other method, this cell line becomes a

11. refers to the stage of the culture after the cells are
isolated from the tissue

12. Example of cell line used in intestinal permeability studies

13. Cell cultures metabolomics can be used for identifying of a
pathological condition

14. %BLANK have the capacity to self-renew and differentiate into
specialized cells and are defined by their origin and degree of
potency

15. %BLANK are derived from the inner cell mass of a pre-implantation blastocyst

16. are isolated from adult sources such as bone marrow and
adipose tissues or perinatal tissues,

17. Cell lines derived from primary cultures have an infinite life span

1. True

2. false

18. E. coli are the primary organism used for producing proteins in
recent times.

1. True

2. False

19. If toxicity testing of new drugs is done in appropriate in vitro cell
lines, limited animal model toxicity tests are needed.

1. True

2. False

20. Control of the environmental factors is easy in cell culture

1. True

2. False

21. Cell culture mimics more closely an in vivo growth environment.

1. True

2. False

22. Transgenic cells have been successfully used for herpes simplex virus
detection

1. True

2. False

23. Stem cells can be used for drug discovery and development.

1. True

2. False

24. Transgenic technology in cell culture involves incorporation of stable
genomic materials into the cell

1. True

2. False

25. Differentiate cell strain and cell line

26. Define organ culture ?

27. Define Primary culture?

28. What is Stem cell culture?

29. What is transgenic technology?

30. List out the advantages and limitations of the cell culture?

31. Describe about the applications of cell culture in various fields?