Lesson 1 – Development of Cell Lines: (A) Conventional Approaches exam

1. Find out the reasons why some wild type viral isolates can be easily grown
in primary culture but not in the continuous cell lines.

2. Find out the mechanism of involved in chemical induced transformation

3. Discuss how telomerase enzyme used in immortalization

4. Long term exposure of cellular DNA to short wave UV-Clight can
directly cause dimerization of –

a) Adenine or thiamine

b) Cytosine or guanine

c) Thymine or Cytosine

d) Thymine only

5. Deaminating agents can cause transition mutations by converting:-

a) Thymine to cytosine

b) Uracil to cytosine

c) Cytosine to guanine

d) Cytosine to uracil

6. . Who created the first immortal human cell line?

a) Carrel& Burrows

b) Dr. Gey

c) Harrison

d) None of these

7. Human heptocellular carcinoma is the source of which of the
cancerous cell line?

a) HepG2

b) HeLa

c) CHO-K1

d) BHK-21

8. Which ploidy is obeyed by finite cell line?

a) Aneuploidy

b) Polyploidy

c) Diploidy

d) Euploidy

9. Spontaneously developed chicken cell line is

a) QT 35

b) DF-1

c) MCF

d) A72

10. Some continuous cell lines derived from normal tissue are nontumorigenic, such as .

11. The scientist developed the
Hybridoma technology

12. Serial subculturing of cell lines over time can increase the chances of
and .

13. In HAT medium, HAT stands
for .

14. HGPRT stands for .

15. The chemical commonly used for cell fusion

16. Transformation is a process of alteration of cell phenotype,
presumed to occur via a reversible genetic change.

a) True

b) False

17. Finite Cell lines have slow growth rate and monolayer mode of
growth.

a) True

b) False

18. Polycyclic aromatic hydrocarbon (PAH) is an example of alkylating
agents.

a) True

b) False

18. The hybridomas are made by fusing T cells with myeloma cells.

a) True

b) False

19. Monoclonal antibodies are heterogenous antibodies produced from
single clone of plasma cells.

a) True

b) False

20. Write the difference between primary cell culture and continuous
cell lines.

21. Write in brief about development of cell line from cancerous tissues.

23. What are the differences between finite and continuous cell lines?

24. Mention the conventional methods used for cell line development?

25. What is monoclonal antibody? How they are different from the
polyclonal antibodies?

26. Briefly describe the various methods used for cell line development.

27. Briefly describe difference between primary culture and continuous
cell lines and advantages of established cell line