Lesson 1 – Behaviour of cultured animal cells – Anchorage dependence exam

1. Detail on the requirements of substrate, ECM molecules required,
cellular interactions involved and the mechanism of anchorage
dependence for a neuronal cell culture with relevant illustrations and
figures.

2. Which type of intercellular junction is the least likely to allow
extracellular fluids to move through the space between cells?

a) Desmosome

b) Tight junction

c) Gap junction

d) adherens

3. What is the key role of Chondroitin sulfate proteoglycans? ion!

a) Extracellular matrix interaction

b) Protection to cell

c) Production of cellular enzymes

d) Digesting the toxins

4. Which of the following is found in epithelial cells?

a) Desmosomes

b) Peroxisomes

c) Annulus

d) Integrins

5. Which of the following protein is present in large amount in tight
junctions?

a) Albumin

b) Globulin

c) Claudin

d) Elastin

6. A defect involving the tubulin molecules will affect which of the
following processes?

a) Mitosis

b) Axonal transport in neurons

c) Both of these will be affected

d) None of these will be affected

7. Which of the following is the odd one out?

a) Elastins

b) Collagens

c) Spectrins

d) Proteoglycans

8. Which of the following proteins are abundant in the extracellular
matrix?

a) Tubulin

b) Myosin

c) Collagen

d) Actin

9. Which of the following is correct about integrins?

a) Fibronectin

b) Laminin

c) Both

d) None

10. Which of the following is mitogenic?

a) Fibronectin

b) Laminin

c) Both

d) None

11. Proteoglycans are %BLANK%, transmembrane receptors
%BLANK% signaling domain

a) low affinity, without

b) high affinity, with

c) low affinity, with

d) high affinity, without

12. The gap junction in nerve cells are called as .

13. Protocadherins are abundant in cultures of origin (cell
type)

14. The process by which extracellular messages translate into intracellular
changes is termed .

15. ions is required for the function of cadherins

16. is a transmembrane protein responsible for
anchoring the extracellular matrix (ECM)

17. Cell shape and rigidity is mediated by .

18. Cell to substrate interactions of integrins are mediated through
sequence

19. interaction is observed between cells of different
lineages

20. EDTA is used as a chelating agent

a) True

b) False

21. Hemi desmosomes are present in keratinocytes

a) True

b) False

22. The movement of cell with respect to rigidity is called durotaxis

a) True

b) False

23. The primary messenger molecules bind to the trans membrane
receptors

a) True

b) False

24. Calcium acts as an extra cellular messenger

a) True

b) False

25. Tight junctions are made up of units that are hemophilic in nature

a) True

b) False

26. Fibrocytes secrete type II collagen

a) True

b) False

27. Microtubule assembly is inhibited by vincristine

a) True

b) False

28. Homotypic versus heterotypic cellular interactions

29. Inter cellular junctions in cell culture

30. How different substrates contribute to variations in anchorage
dependence

31. Integrins versus Cadherins with respect to anchorage dependence

32. Commercially available ECM

33. Synthetic ECM

34. Role of serum in anchorage dependence

35. Importance of cell adhesion molecules in cell culture

36. Role of ECM in anchorage dependence