Lesson 5 – FISH & chromosome painting exam

1. What are the different types of FISH techniques used and briefly
describe about them?

2. What is the difference between chromosome painting and FISH?

3. How will you apply FISH technique for the study of genetic
abnormalities in animals?

4. What does FISH detect?

Protein structure abnormalities

Specific chromosome copy number aberrations

Presence of specific antigens

Presence of complement

5. Where does the FISH probe localize to

Golgi apparatus

Cytoplasm

Cell membrane

Nucleus

6. What is the FISH probe composed of

Proteins

Lipids

Carbohydrates

Nucleic acids

7. What is the maximum number of FISH probes that can be used in a
single experiment

Two

Three

Four

Five

8. Which of the following does not affect the formation of hybrid DNA

Ionic strength Three

Pressure

Temperature

Homologous DNA

9. What is a probe?

Chemically synthesized DNA

Purified DNA

Fragmented DNA duplex

Either purified or synthesized single stranded DNA

10. Why is a probe labeled?

Improve visibility

Improve stability

Improve location identification

Improve binding capability

11. What is the function of polynucleotide kinase?

Addition of γ – phosphate at 3’ – OH

Addition of γ – phosphate at 5’ – OH

Removal of γ – phosphate at 3’ – OH

Removal of γ – phosphate at 5’ – OH

12. In how many ways a DNA can be labeled?

2

3

4

5

13. Which substrate can be used for alkaline phosphatise enzyme

PNPP

BCIP

NBT

All of the above

14. Fluorescence microscopy was developed by .

15. FISH was developed by .

16. The enzyme for the substrate is .

17. is a high resolution FISH technique

18. Comparative chromosome painting is called .

19. FISH is used to detect tumor

20. RISH can detect genes.

21. combines fluorescence microscopy, CCD-imaging and
Fourier spectroscopy

22. OPD is used as a substrate for enzyme

23. FISH used filter-based system where separate images are
acquired sequentially for each fluorochrome used.

24. In fluorescence light is emitted at higher wavelength.

True

False

25. FISH can be used in interphase chromosome also.

True

False

26. Triton X-100 to remove lipid membrane of cells.

True

False

27. Prehybridzation process ensures specific binding of only the target
sequence and probe sequence.

True

False

28. Probe can be amplified by molecular cloning or by PCR.

True

False

29. Probe can be denatures by extreme pH.

True

False

30. In cyctosine base modification can be done at C5 and N6 position.

True

False

31. Formamide reduce the melting temperature of hybridization.

True

False

32. Chromosome painting allows visualization of individual
chromosomes in metaphase or interphase

True

False

33. Mitotic inhibitors inhibit FISH

True

False