Lesson 5 – Maintenance of Cell Lines exam – Brihha Assessment Platform

1. In cell culture techniques why the passage number of the cell line is
always tried to maintain at low level during culture. How the passage
number can be maintained at lowest level.

2. What is the composition and use of maintenance medium in cell
culture?

3.Which of the following is a transformed cell line?

a) CHO-K1

b) HeLa

c) Mouse fibroblasts

d) 293-T

4. On which of the factors the maintenance of cell lines does not depend?

a) Type of cell line

b) Growth rate

c) Metabolism

d) None of these

5. Which enzymes can be used for dissociation of adherent cells?

a) Pronase

b) Dispase

c) Both a) and b)

d) Lyzozyme

6. Which is the appropriate phase for subculturing of cells?

a)Late log phase

b) Stationary phase

c) Any phase

d) Mid log phase

7. Attachment of cells to monolayers is because of:

a) Dispase

b) Surface glycoproteins

c) Ca2+

d) Both b) and c)

8. Which solution is used first for rinsing themonolayer if the cells are
difficult to remove?

a)DPBS

b) Serum

c) Trypsin-EDTA

d) Both a) and c)

9. Which of the following is not an attachment factor?

a)Fibronectin

b) Collagen

c) Pronase

d) Poly-L-lysine

10. The synthetic ECM coated plate contain

a) Fibronectin

b) Collagen

c) Pronase

d) Poly-L-lysine

11. The concentration of the cells is directly proportional to:

a) Frequency of medium change

b) Attachment factor

c) Both a) and b)

d) None of these

12. Higher dissolved oxygen concentration in the culture media are toxic
and lead to

A)DNA degradation

B)lipid peroxidation

C)C) Metabolism of nutrients in culture media at a rate greater than that required for consumption

D)all of the above

13. Split ratio for established cell line ranges from to
.

14. ATCC stands for .

15. is a subpopulation of a cell line positively selected from
the culture by cloning or some other method.

16. Cell line becoming immortal through a process is called
.

17. percentage of trypsin is commonly used for subculturing
of cell lines.

18. Finite cell line divides indefinitely.

True

False

19. Fibroblast cells are polygonal in shape with more regular dimensions,
and grow attached to a substrate in discrete patches.

True

False

20. Subculture allows further propagation of the cell line.

True

False

21. Cell scrappers are used for subculturing of non-adherent cells.

23. The stage of the culture after the cells are isolated from the tissue
and proliferated under the appropriate conditions until it reach
confluence is known as a primary culture.

24. The subculture ratio is in between 6-10.

25. What is cell bank?

26. Explain the subculturing of adherent cells.

27. Explain in brief the factors to be kept in mind before subculturing.

28. How the suspension cells is subcultured? Explain with the help of a
diagram.

29. Write the method of handling and maintenance of subculture records.

30. Write briefly about trypsin inhibitors.

31. What are the precautions should be taken during cell line maintenance?

32. What are the factors affecting the cell line growth?