Lesson 4 – Characterization of Cell Lines exam

1. How adherent cell culture can be adapted to suspension cultures?
Explain with examples.

2. What types of expression vector systems are currently available
commercially for large scale production of biopharmaceutical
products?

3.Which technique is used for DNA profiling:

A. Microarray

B. Immunohistochemistry

C. PCR

D. Chromosome banding

4. Sky technique related to :

A. Isoenyzme pattern

B. Immunophenotyping

C. Western blot staining

D. Karyotyping

5. EPC stands for %BLANK%

A. End of Production Cells

B. End of Production Cultures

C. End of Production of Contamination

D. None of above

6.Chromosomal analysis also referred to as %BLANK% is one among the
simplest traditional methods for distinguishing among species.

A.Karyotyping

B.Chromosome painting

C.Chromosome Banding

D.All of the above

7. MHC stands for %BLANK%

A. Major Hysto compatibility complex

B. Minor Histo compatibility complex

C. Major Hitso compatibility complex

D. Minor Hytso compatibility complex

8. %BLANK% marks epithelial cells and mesothelium

A.GFAP

B.Cytokeratin

C.FISH

D.MHC

9.Q-banding involves the use of the fluorescent dye :

A. Giemsa dye

B. Methylene Blue

C. Quinacrine

D. Both A and C

10. %BLANK% is an informative approach to genetic identification.

A. STR

B. VNTR

C. PCR

D. Both B and C

11. Western blotting is based on the principle of %BLANK% where proteins
are separated into polyacrylamide gel according to their molecular weight.

A. Immunologicalproteins

B. Immunotherapy

C. Radiography

D. immunochromatography

12. %BLANK% is also a good diagnostic test and is quicker than
chromosomal analysis.

A.Electroporation

B. Isoenzyme electrophoresis

C. Western blotting

D. FISH

13. In G- banding rich DNA stains dark.

14. VNTR stands for .

15. dye is used in Q-banding

16. DNA fingerprinting is based on identification of
and regions of
hypervariability of chromosome.

17. Senescence refers to the process by which a cell line is found to contain
chromosomes characteristics of another cell line due to crosscontamination. Is this true or false?

True

False

18. All cancer cells contain transformed cell but all transformed cells do not
contain cancer cells

True

False

19. The inverted microscope is one of the most important tools in the tissue
culture laboratory.

True

False

20. In Q- Banding AT-rich pairs decreases the fluorescence, GC pairs
increase the fluorescence.

True

False

21. C-banding can be used to specifically stain constitutive heterochromatin
regions.

True

False

22. What is the need of cell line characterization?

23. Describe antigenic markers used for cell line characterization.

24. What are the DNA based methods used for cell line characterization?

25. Explain chromosome Banding?

26. What are the drawbacks of morphological analysis based cell line
characterization ?

27. Briefly describe different methods of cell line characterization.

28. Define the term lineage marker? Explain each of them?