Lesson 2 – Development of Cell Lines: (B) Immortalization or Transformation of Cells by in vitro Genetic Manipulation exam

1. Search literature and make a list of proteins (genes) which can
influence (up-regulation or down-regulation) the cell cycle
regulatory proteins (check points) in human cells

2. . Describe different components of a suitable mammalian expression
vector which can be used for development of an immortalized, stable
cell line

3. Describe the most preferred viral vectors commonly used for cell line
development and discuss their advantages and disadvantages.

4. p53, pRb are an example of -

a) Tumor suppressor gene

b) Oncogene

c) pseudogene

d) housekeeping gene

5. Following transfection of cells with SV40 LT transgene the LT
antigen interact with –

a) Rb gene

b) P53 gene

c) P57 gene

d) Rb and P53 genes

6. HEK 293 cell line, was immortalized by

a) SV40 LT

b) HPV E6 & E7

c) hTERT

d) Human Adenovirus 5 E1A and E1B

8. EBV can be used to transform the following primary culture cells –

a) Fibroblast

b) Epithelial cell

c) Lymphocytes

d) All of the above.

9. Cells which have undergone transformation frequently become

a) anchorage independent

b) anchorage dependent

c) stable

d) unstable

10. Which of the following is the characteristic of a normal cell?

a) Anchorage independent

b) Immortal

c) Dependent on external growth factor

d) No contact inhibition

11. Lentiviral vectors can integrate into the genome of -

a) Dividing cells

b) Non-dividing cells

c) Both

d) None

12. Telomerase activity is detected in

a) Primary Culture cells

b) Somatic cells

c) Germ cells

d) Finite cell lines

12. Phenotypic changes that do not involve alterations in the DNA
sequence are known as changes.

13. hTERT stands
for

14. Examples of anti-apoptotic genes are

15. Activated oncogenes causes cells to proliferate instead of
undergoing .

16. Human papilomavirus (HPV) and
genes have the ability to immortalize primary cells.

17. diploid human cell line.

18. Uncontrolled expression of the cell cycle regulating genes can cause
cancer.

True

False

19. Ploidyof chromosome does not change after transformation.

True

False

20. hTERT is a ribonucleoprotein that prevents elongation of telomeres.

a) True

b) False

21. 4. VP1 protein of SV 40 virus is used to immortalize the cell line.

a) True

b) False

22. A mutated p53 gene may contribute to uncontrolled cellular
proliferation

a) True

b) False

23. All immortal cell lines are cancerous.

a) True

b) False

24. hTERT immortalized cells are phenotypically similar to the original
cells.

a) True

b) False

25.Mention different viral and non-viral genes that are used for
development of continuous cell line.

26. How cell cycle regulating genes are involved in cellular
immortalization?

27. Describe immortalization of cell line by genetic engineering method
with a suitable example

28. Write a short note on proto oncogenes and tumor suppressor genes.

29.Describe very briefly about different methods used for
immortalization of cultured cells

30. How different oncogenic viruses can be used to develop continuous
cell line.