Lesson 5 – Contact inhibition and changes in the behaviour of animal cells upon culture exam

1. Prepare a flash card learning aid illustrating how substrate variation can
influence the behaviour of cultured animal cells. You can form groups and
groups can work on different substrates for different culture systems. One
side of the flash card should have a representative picture and the other
side should have textual information

2. Contact inhibition is characterized by
i. cessation of motility of cells
ii.random orientation
iii.ruffling of cell membrane

a) i and ii

b) ii and iii

c) i, ii and iii

d) i and iii

3.Saturation density reached by transformed cells during plateau
phase is

a) lower than that of the finite counterpart cells

b) higher than that of the finite counterpart cells

c) equivalent to that of the finite counterpart cells

d) none of the above

4. Loss of specific cell-to-cell interactions and adhesion can be reverted
through

a) provision of growth factor

b) treatment of growth surface

c) ECM

d) all of the above

5. Doubling time of cultured cells follow which of the following pattern
(from shortest to longest)

a) primary culture < finite cell line < transformed cell line

b) finite cell line < transformed cell line < primary culture

c) transformed cell line < primary culture < finite cell line

d) transformed cell line < finite cell line < primary culture

6. Deamination of glutamine in culture medium leads to built up of

a) ammonia

b) lactic acid

c) asparagines

d) alkaline pH

7. When cultured cells undergo transformation, they undergo
phenotypic changes associated with this genetic instability that
include

a) aberrant growth control

b) immortalization

c) malignancy

d) all of the above

8. Dedifferentiation is an %BLANK% loss of the specialized
properties that those cells would have expressed in vivo

a) irreversible

b) reversible

c) both

d) none of the above

9. Which of the following is not a property of suspension culture

a) steady state of culture requirements

b) anchorage dependence

c) density limitation of growth

d) anchorage independence

10. The velocity of migration of cells is proportional to the
number of cells with which they are in contact

11. The normal cellular growth genes that under certain conditions get
converted into oncogenes are called as

12. The inhibition of mitosis of cultured cells associated with an increase in
cell density is termed as

13. While primary cultures are (ploidy), continuous cell lines
are

14. . The drop in the pH of the culture medium is due to accumulation of
produced by from glucose

15. Primary metabolites are produced during the and
secondary metabolites during the of the culture

16. Cessation of cellular proliferation due to aging is termed as

17. Contact inhibition is a property exhibited by suspension cultures

a) True

b) False

18. Transformed cell lines are immortal

a) True

b) False

19. Density limitation of cell proliferation is exhibited more intensely by
fibroblasts than endothelial cultures

a) True

b) False

20. Primary cultures are homogenous with respect to their cell
population

a) True

b) False

21. Homeostatic regulation and histological architecture that prevailed
in vivo are shed off when cells are cultured under in vitro

a) True

b) False

22.The rate of change of pH of culture medium is dependent on the cell
concentration

a) True

b) False

23.Growth on semi solid medium is a characteristic of transformed cells

a) True

b) False

24. Cultures derived from embryonated tissues reach senescence much
earlier than those from adult tissues

a) True

b) False

25. 1. Mechanism of contact inhibition

26. Density limitation of proliferation in different culture systems

27. Cellular metabolism in cultured cells

28. Compare and contrast senescence with immortalization

29. Influence of substrate variation on the behaviour of cultured cells