Lesson 3 – Growth pattern of cultured animal cells exam

Welcome to your Lesson 3 - Growth pattern of cultured animal cells exam

1. Construct a growth curve with a seeding density of 2 x 106
cells/ml, 36
hours of lag phase, peak cell concentration of 3 x 107
cells/ml on day 9 that
remains a plateau thereafter. Determine the population doubling time,
saturation density of this suspension culture and comment on the growth
profile of this culture.
2. Adaptation is the characteristic feature of phase of
the growth curve
3. During trophophase, cell growth is due to excess of
nutrient availability in the culture environment
4. is an example of a growth limiting substrate
5. Undifferentiated self-renewing cells with potential for high plasticity are
called as %BLANK% .
6. Provision of more surface area than volume to increase cell numbers
during log phase can be achieved by method by
immobilizing cells to an inert bead
7. Cryopreservation is best achieved when cells in the phase
are harvested for storage
8. Longer lag phase is indicative of inoculation density
9. The cell organelle involved in apoptosis is %BLANK% .
10. Lag phases exhibits no change in cell number but an increase in mass
12. Senescence refers to the process by which a cell line is found to
contain chromosomes characteristics of another cell line due to
cross-contamination.
13. The specific growth rate of cells is independent on the concentration of nutrients in the medium.
14. Transformed cell lines are characterized by short doubling times
15. Growth curve of healthy cultured cells follows a sigmoid pattern
16. Differentiation of cultured cells can be observed during the lag phase
of growth
17. The growth promting capacity of a medium is indicated by the
population doubling time measured during the mid plateau phase
18. Growth of cells in primary culture continue to be in exponential
phase after reaching confluency
19. Plateau phase of growth curve
20. Difference between generation time and population doubling time
21. Compare and contrast between growth characteristics of primary culture and
transformed cell lines
22. Seeding density versus saturation density
23. Significance of growth curve analysis
24. Factors influencing the growth pattern of cultured animal cells
25. How would you test the efficacy of anti oxidant property of curcumin for
application in cancer therapy (Clue : Use at different concentrations)