Lesson 6 – Cell culture reagents exam – Brihha Assessment Platform

1. Polymer which provides a positively charged culture surface for the
negatively charged ions of the cell membrane to bind

a. poly lysine

b. polystyrene

c. both a and d

d. polyhistidine

2. Example for balanced salt solutions

a. DPBS

b. HBSS

c. HPEES

d. both a and b

3. Percentage of inclusion levels of DMSO and glycerol for
cryopreservation of cells

a. 5-10

b. 10-20

c. 5-8

d. 1-5

4. Elastase is commonly as dissociating agent for tissues like

a. kidneys

b. lungs

c. liver

d. heart

5. The concentration of trypsin in cell culture can range from

a. 1-2%

b. 5-10%

c. 0.025% to 0.5%

d. 2-5%

6. Compare to other cryoprotectant is less toxic in high
concentration

7. Commonly used cryoprotectants in cell culture are and

8. Normal attachment, growth and development of many cell types are
dependent on and

9. These media are generally specifically formulated to support the
culture of a single cell type

10. is most commonly used for detachment of
adherent cell lines and dissociation of tissues

11. The common extracellular matrix proteins used for coating are
collagen and fibronectin.

1. True

2. False

12. The concentration of trypsin in cell culture can range from 1% to
2%

1. True

2. False

13. Balanced salt solution Helps to maintain osmolarity in cell culture

1. True

2. False

14. HEPES is an example for buffer used in cell culture

1. True

2. False

16. Explain the process of cryopreservation of cell line ?

15. Glutamine is not an critical amino acid supplement in cell culture

1. True

2. False

17. Explain the dyes used in cell culture ?

18. Explain about common cell culture adhesion reagents?

19. Explain in detail the various cell culture reagents and their applications?