Lesson 2 – Different types of cell culture media exam

1. Complete medium consists of :

a. Basal medium with calf serum

b. Basal medium without calf serum

c. Both of the above

d. None of the above

2. Anchorage dependent cells are:

a. Adherent cells on the substrate

b. Cells in suspension

c. Both of the above

d. None of the above

3. The media generally formulated to support culture of sinfle
cell type

a. Basal media

b. Protein free media

c. Serum free media

d. Synthetic media

4. Commonly used antifungal drug in cell culture

a. PenicillinG sodium,

b. Chloromycetin

c. Tetracycline

d. Amphoterici

5. Example for basal media

a. RPMI-1640

b. IMDM

c. F-10

d. DMEM

6. plays an important role in attachment of cells.

7. is an iron transport protein that supplies iron to the cell
membrane.

7. Aprotinin inhibits

9. Vitamin E is present in media

10. media contain contamination-free ultra pure inorganic and
organic ingredients

11. Calcium is reduced in suspension cultures in order to minimize cell
aggregation and attachment.

1. True

2. False

12. Eagle’s MEM contains only the fat-soluble vitamins

1. True

2. False

13. Antibiotics hide the presence of low-level, cryptic contaminants

1. True

2. False

14. Phenol red, a pH indicator, is added to medium to colorimetrically
monitor changes in pH.

1. True

2. False

15. Protein free media is also referred as defined culture media (FALSE

1. True

2. False

16. Describe about types of media and give examples for
commercially available media?

17. Write the complete composition of commonly used
commercial media?

18. Describe each types of media and each of its constituents in detail ?