Lesson 1 – Introduction to cell culture media exam

1. Osmolarity is the:

(a) Measure of depression in the freezing point

(b) Elevation of the vapour pressure

(c) Both of the above

(d) None of the above.

2. To maintain viscosity the substance added in cell culture is:

(a) Serum

(b) Carboxymethyl cellulose

(c) Polyvinyl pyrollodine

(d) All of the above.

3. The pH of the medium in which cells are growing, may fall
because of:

(a) Production of CO2

(b) Production of lactic acid

(c) Both of the above

(d) None of the above.

4. The optimum pH for cell growth

(a) 7.6

(b) 7.4

(c) 6.5

(d) 6

5. Cryopreservation of cells is done at following temperature

(a) -56°c

(b)-80°c

(c)-196°c

(d)-200°c

6. The acceptable osmolality for most of the cells is

7. is the indicator of pH in cell culture media.

8. Temperature recommended for most of the human cell lines is

9. Addition of helps to prevent foaming.

10. A is a liquid or gel designed to support the growth of
cells.

11. HEPES is a weak buffer in the pH 7.2–7.6 range

1. True

2. False

12. The viscosity of culture medium is not mainly by the serum content

1.True

2. False

13. Osmolality is usually measured by depression of the freezing point or
elevation of the vapor pressure of the medium

1. True

2. False

14. Ham’s F-10 is suitable for growth of the suspension cells, mainly for
lymphoid cells

1. True

2. False

15. Carbon dioxide in the gas phase dissolves in the medium and
establishes equilibrium with HCO3 ions, and lowers the pH.

1. True

2. False

16. Explain the major historical events in development of cell culture media

17. Explain the physico chemical properties of cell culture media

18. Write down the common cell culture media used for different type of cells?