Lesson 8 – Suspension and adherent cell cultures exam

1.The following is used as dissociation of cells

(a) Trypsin

(b) Balanced salt solution

(c) Growth medium

(d) none of the above

2. Suspension cells also known as

(a) Anchorage dependent

(b) Anchorage independent

(c) Both a and b

(d) None of the above

3. The following is the characters of suspension culture.

(a) trypsin treatment is not required

(b) culture is diluted and expanded

(c) The depth of medium in static cultures - 2−5 mm.

(d) All of the above

4. The following is example of adherent culture

(a) Fibroblast cell type

( b) Tumor cell

(c) Hematopoietic cell

(d) All of the above

5.The following is the growth properties of adherent cells

(a) Contact inhibition

(b) cell interaction

(c) Diffusion boundaries

(d) All of the above

6.Example for media developed for Lymphoid cells is

(a) Fischer’s media

(b) DMEM

(c) MEM

(d) All of the above

7. Cultural requirements for suspension culture are

(a) Cyclic maintenance

(b) enzymatic disaggregation

(c) volume and dilution

(d) All of the above

8.Criteria for subculture are as follows

(a)Cell concentration

(b) pH

(c)Time since last subculture

(d) all of the above

9. medium particular has designed for suspension culture.

10. The cells are anchorage dependent and propagate as a
monolayer.

11. Cell concentration, which should not exceed cells/mL for
most suspension-growing

12. cells do not attach to the surface of the culture vessels and
can be grown floating in the culture medium.

13. When the cells are near the end of exponential growth i.e. %
confluent they are ready to be subcultured

14. and cells can be grown in suspension form.

15. The ion concentration is less in RPMI medium used for suspension
culture

16. Progression of cell dissociation can be analysed by

17. Suspension culture is useful for bulk production & batch processing

1. True

2. False

18. Stirrer culture is used for adherent cell culture

1. True

2. False

19. Suspension culture is applicabe epithelialcells

1. True

2. False

20. RPMI 1640 and fischer’s media were developed for Lymphoid cells

1.True

2. False

21. In adherent cells subculture is quicker and less traumatic for the cells,
and scaleupis easier

1. True

2. False

22. Ph of media declines as the cell concentration rises

1. True

2. False

23. Fragile culture such as endothelium can be subcultured at around
5x10⁵cells/ml

1. True

2. False

24. Murine ascites tumors are examples of cells that grow continuously in
suspension

1. True

2. False

25. DIFFERENTIATE MONOLAYER AND SUSPENSION CULTURE?

26. DESCRIBE ABOUT BASIC CELL TYPES GROWN IN CELL CULTURE?

27. DESCRIBE ABOUT THE CRITERIA REQUIRED FOR SUBCULTURE?

28. WHAT ARE ROLLER BOTTLES AND THEIR IMPORTANCE IN PROPOGATION
OF SUSPENSION CELLS?

29. DESCRIBE ABOUT PROPOGATION OF SUSPENSION CELLS?

30. List adherent cell lines and their applications?

31. Explain the procedure of subculturing monolayer adherent cell
culture