Lesson 7 – Commonly used cell lines and their uses exam

1. Cell lines are used for the:

(a) Growth of different viruses

(b) Production of vaccine

(c) Gene expression study

(d) All of the above.

2. CHO cell line:

(a) Has been derived from adult Chinese hamster ovary

(b) Used for the generation of tissue plasminogen activator (tpa)

(c) Both of the above

(d) None of the above.

3. Animal cell culture are used for large scale production of:

(a) Different vaccines used in human and animals

(b) Different therapeutics used for the treatment of human and animal diseases

(c) Both of the above

(d) None of the above.

4. The genes expressed by C-127 cell cultre system is.

(a) HBsAg

(b) HSVgD&9B

(c) EBVgP40

(d) HIVgp160

5.The origin of CHO-K1 cell line is

(a) Ovary

(b) Lung

(c) kidney

(d)Liver

6. the possible causes of misidentification are

(a) Importation of authenticated stock.

(b) Failure to count cells at each subculture.

(c) Handling more than one cell line at a time

(d)both b and c

7.Example for tissue markers

(a)MHC group antigens

(b) intermediate filament proteins

(c)cytokines

(d)both b and c

8.DNA profiling can be done by

(a)STRanalysis

(b)DNA barcoding

(c)enzyme analysis

(d)DNA banding

9. Once a primary culture is subcultured, the culture becomes a

10. developed small pox vaccine by collecting skin of calves or
sheep affected with small pox.

11. cells has been used for the reproduction of canine distemper
vaccine

12. Vero cell line is obtained from

13. The origin and species of HeLa cell line is

14. Cell Line used for the expression of hepatitis B surface antigen
(HbsAg) and interleukin-2

15. Analysis of gene provides reliable species identification
of animal cell lines

16. and are the most useful techniques available for cell
line characterization facilitated by the abundance of antibodies.

17. WI-38 is a finite cell line, from mouse lung cells

1. True

2. False

18. Various cell lines help in the study of pattern of release of different
microorganisms

1. True

2. False

19. 3T3-A31 is a continuous cell line from mouse normal tissue

1. True

2. False

20. The toxic effect of various pharmaceutical compound cannot be known
by cell culture

1. True

2. False

21. Poliovirus vaccine in the Salk Institute was developed using mouse kidney
cell culture

1. True

2. False

22. Lineage markers are helpful in establishing the relationship of a particular
cell line to its tissue of origin

1. True

2. False

23. unique markers are used distinguish among cell lines from the same
tissues but different donors

1. True

2. False

24. Trypsinization is required for quinacrine banding

1. True

2. False

25. List the usage of cell lines in vaccine production

26. Define characterisation of cells?

27. Describe about phenotypic characterisation of cells?

28. Describe about chromosome banding?

29. List the possible causes of cross contamination?

30. List 15 COMMONLY used cell lines, their source and their
applications?

31. Describe about various techniques employed for analysing
characteristics of cells?