Lesson 6 – Continuous cell lines exam – Brihha Assessment Platform

1. The following is a property of continuous cell line

(a) Aneuploid

(b) High growth rate

(c) both of the above

(d) none of the above

2. The appearance of a continuous cell line is marked by an alteration in
cytomorphology

(a) Cells are smaller

(b) Less adherent

(c) Higher ratio of nucleus to cytoplasm

(d) all of the above.

3. Disadvantages of Continuous cell lines are :

(a) Genetically unstable and heterogenous

(b) Low serum required

(c) both a & b

(d) Faster growth rate

4. The viral oncogene which is involved in cell line transformation are

(a) Retrovirus

(b) Adenovirus E1a

(c) Epstein−Barr virus

(d) All of the above

5. The markers of continuous cell line are:

(a) Chromosomal

(b) Enzymatic

(c) Antigenic

(d) all of the above

6.cell type of VERO cell line

(a) Fibroblast

(b) Epithelial

(c) lymphoblast

(d) none of the above

7.doubling period of transformed cells will be

(a) 24 h-36 h

(b) 36 h-48 h

(c)12 h-36 h

(d)5 h-6 h

8.Immortalisation can be achieved by following methods

(a) mutagens

(b)viral infection

(c)oncogenes

(d)all of the above

9. Immortalization is a process involving the inactivation of a number of
cell cycle regulatory genes, such as and .

10. Full form of CHO cell line

11. Normal cells divide only a limited number of times before losing their
ability to proliferate, these cell lines are known as .

12. The origin of 3T3 cell line is

13. Retroviruses express activated oncogenes

14. cell line was developed from human cervical cancer cells

15. Namalwa cell line is used for production

16. Oncogenes which override the activity of the senescence genes by re
expressing

17. HeLa cells, are tumor derived cell line, from a cervical cancer.

1. True

2. False

18. The continuous cell line requires cells to go through a process called
transformation

1. True

2. False

19. The yield of continuous cell line is low

1. True

2. False

20. It is less likely to lose normal phenotype in continuous cell line

1.True

2. False

21. The activity of telomerase is higher in immortal cell lines and sufficient
to endow the cell line with an infinite lifespan

1. True

2. False

22. Continuous cell lines are genetically unstable and homogenous

1. True

2. False

23. vero cell line is commonly used for human vaccine production

1. True

2. False

24. Adenovirus infection is an especially effective technique of
immortalizing cells.

25. List advantages and disadvantages of cell lines ?

26. List properties of continuous cell lines?

27. Describe about senescence and its inference to continuous cell lines?

28. Describe about immortalisation by retro virus infection?

29. LIST COMMONLY AVAILABLE CELL LINES AND THEIR APPLICATIONS?

30. DESCRIBE ABOUT DIFFERENT METHODS OF IMMORTALISATION OF
CELLS?