Lesson 4 – Primary cell culture exam – Brihha Assessment Platform

1. In Trypan blue dye exclusion test:

(a) Viable cells take blue colour, whereas dead cells remain unstained

(b) Dead cells take blue colour, whereas viable cells remain unstained

(c) Both viable and dead cells take blue colour

(d) Both viable and dead cells remain unstained.

2. Separation of cells from organ/ tissue for cell culture may be done:

(a) By using different proteolytic enzymes

(b) By different mechanical means

(c) By using different chelating agents

(d) All of the above.

3. Cells for primary monolayer cell culture is derived

(a) Directly from organ/tissue of live animal

(b) Directly from other cell culture tube

(c) Directly from transformed cells

(d) All of the above.

4. Separation of cells are done by using

(a) Trypsin

(b) Versene (EDT A)

(c) Trypsin + Versene mixture

(d) All of the above

5. Sodium versenate acts by :

(a) Chelating action

(b) Proteolytic action

(c)Mechanical action

(d) All of the above.

6. The separation of cells by chelating action of sodium versenate was
described by :

(a) Van Wezel

(b) Bodian

(c) Zwilling

(d) Pizarro

7. Applications of primary cell culture

(a) Vaccines production

(b) genetic engineering

(c) Drug discovery

(d) All of the above.

8. Primary cells can be exposed to --------- to attain immortalization

(a) radiation,

(b) chemicals

(c) viruses

(d) All of the above

9. refers to culture of cells obtained directly from the
tissues or organ collected aseptically from different apparently healthy
animals

10. Blood and lymph contains a range of cells in form

11. Generally % solution of crude trypsin or % pure
trypsin having pH 7.2- 7.4 at 4 to 37°C is commonly used for dispersion of
cells.

12. Dispersion by chelating agent is used as 0.02% solution in
buffer saline lacking Ca and Mg saIt at pH 7. 2 to 7. 4.

13. Commonly used method in tissue culture laboratory to test the viability of
cells is test

14. Growth of the cell monolayer depends upon the and
time of the cells.

15. have also been used extensively in 3D bioprinting applications.

16. MTT assay is used to study the of new drugs (to study the effect
and safe dosage) and/or drug carriers

17. What is primary cell culture?

18. List advantages of primary cell culture?

19. List some applications of primary cell culture ?

20. How can primary cells be used in cancer research ?

21. What is the role of primary cell culture in Stem Cell Therapy?

22. Describe about mechanical and chemical disaggregation of tissue

23. Explain PRIMARY CELL CULTURE TECHNIQUE ?