Lesson 2 – History of cell culture development exam

Welcome to your Lesson 2 - History of cell culture development exam

1. Use of antibiotics in cell culture was introduced by:
2. HAT medium used for the selection of fused hybrid cells in hybridoma
culture was introduced by:
3. Use of trypsin to free cells from the tissue matrix for cell culture was
described by:


4. Subculture of fibroblastic cell lines was introduced by:
5. Implants can be made from:
6. The basis for the improvement of cell culture method was laid by
7. %BLANK% cultivated chick embryo using chicken clot plasma
8. %BLANK% first described cell culture in a nutrient medium containing glucose,
peptone, and different salts.
9. Father of the tissue culture is
10. HeLa cell line was derived from
11. The Limited replication capacity of cells in the culture is referred to effect
12. established the first widely used chemically defined
medium
13. introduced proteolytic enzyme trypsin for the subculture
of adherent cells
14. is a fragment of tissue transplanted from its original site and
maintained in an artificial medium.
15 . are devices or tissues that are placed inside or on the surface of
the body.
16. is the use of a combination of cells, engineering materials, and
suitable biochemical factors to improve or replace biological functions
17. Harrison cultivated mouse nerve cells in a lymph clot held by the
hanging drop Method
18. In 1943, Earle et al established the L-cell mouse fibroblast; first
continuous cell line
19. Carrel developed plaque assay for animal viruses using confluent
monolayers of cultured cells.
20. Burrows succeeded in long term cultivation of chicken embryo cell in
plasma clots
21. Growth of virus in cell culture is done by Canti
22. Implants cannot be made from ceramics
23. Addition of antibiotics makes cell culture more difficult
24. The hanging drop culture is a small drop of liquid, such as plasma
26. Differentiate implants and explants
27. Explain hanging drop technique
28. Define Tissue engineering. Defined as the use of a combination of
cells, engineering materials, and suitable biochemical factors to improve
or replace biological functions
29. Define Organ printing.
30. Difference between natural media and synthetic media
31. Write briefly about the technical innovations in Development of Cell and
Tissue Culture